Gentodenz (Density gradient medium for cell separation) - 250 grams

Gentodenz® is a non-ionic tri-iodinated derivative of benzoic acid with three aliphatic hydrophilic side chains. The systematic name of Gentodenz is 5- (N-2, 3-dihydroxypropylacetamido)-2, 4, 6-tri-iodo-N, N'-bis (2, 3 dihydroxypropyl) isophthalamide.

Gradient solutions for the preparation of essentially iso-osmotic Gentodenz gradients has been devised and these can be prepared using an iso-osmotic solution of Gentodenz which contains 27.6% (w/v) Gentodenz (density = 1.15 g/ml) made up in buffered medium. This solution may be diluted to desired concentration by using a buffered diluent containing either sucrose or NaCI as osmotic balancer.

The composition of these diluents are as folIows:

• 0.75 g NaCI or 7.45 g sucrose
• dissolved in 100 ml 5 mmol/l Tris-HCI (pH 7.5) containing 3 mmol/l
• KCI and 0.3 mmol/l CaNa2 EDTA.
• The relationship between density and refractive index (η) can be formulated:
• NaCI diluent Sucrose diluent
• Density = 3.287 η - 3.383 Density = 3.410 η - 3.555